골막기원세포에서 발현되는 혈관내피세포성장인자 관련 자가성장
VEGF-RELATED AUTOCRINE GROWTH IN PERIOSTEAL-DERIVED CELLS
박봉욱, 이성균, 하영술, 김덕룡, 조영철, 성일용, 김욱규, 김종렬, 변준호,
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박봉욱 ( Park Bong-Wook ) - 경상대학교 의학전문대학원 구강악안면외과학교실
이성균 ( Lee Sung-Gyoon ) - 경상대학교 의과대학 구강악안면외과학교실
하영술 ( Hah Young-Sool ) - 경상대학교병원 임상의학연구소
김덕룡 ( Kim Deok-Ryong ) - 경상대학교 의학전문대학원 생화학교실
조영철 ( Cho Yeong-Cheol ) - 울산대학교 의과대학 구강악안면외과학교실
성일용 ( Sung Iel-Yong ) - 울산대학교 의과대학 구강악안면외과학교실
김욱규 ( Kim Uk-Kyu ) - 부산대학교 치의학전문대학원 구강악안면외과학교실
김종렬 ( Kim Jong-Ryoul ) - 부산대학교 치의학전문대학원 구강악안면외과학교실
변준호 ( Byun June-Ho ) - 경상대학교 의학전문대학원 구강악안면외과학교실
KMID : 0355620090350050294
Abstract
Purpose: The development of a microvascularization is important for the homeostasis of normal bone. Vascular endothelial growth factor (VEGF) is one of the most important factors in vessel formation. The purpose of this study was to examine VEGF-related autocrine growth in periostealderived cells.
Materials and methods: Periosteal-derived cells were obtained from mandibular periosteums and introduced into the cell culture. After passage 3, the periosteal-derived cells were further cultured for 21 days in an osteogenic inductive culture medium containing dexamethasone, ascorbic acid, and β-glycerophosphate.
Results: The expression of four VEGF isoforms and VEGFRs was observed in periosteal-derived cells. Treatment with cultures with VEGFR-1 and VEGFR-2 Kinase Inhibitor inhibited osteoblastic differentiation and alkaline phosphatase (ALP) activity of periosteal-derived cells. In addition, exogenous VEGF treatment increased calcium content in the periosteal-derived cells.
Conclusion: These results suggest that VEGF might act as an autocrine growth molecule during osteoblastic differentiation of cultured human periosteal-derived cells.
키워드
Periosteal-derived cells;Vascular endothelial growth factor;Autocrine growth
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